汽車總動員主題曲是《Real Gone》。

另外，附加說明其他插曲如下。

1、Route 66 - Chuck Berry

2、Life Is A Highway（奧斯卡獲獎作品） - Rascal Flatts

3、Behind The Clouds - Brad Paisley

4、Our Town - James Taylor

5、Sh-Boom - The Chords

6、Route 66 - John Mayer

7、Find Yourself - Brad Paisley

8、Opening Race

9、McQueen’s Lost

10、My Heart Would Know - Hank Williams

11、Bessie

12、Dirt Is Different

13、New Road

14、Tractor Tipping

15、McQueen And Sally

16、Goodbye

17、Pre-Race Pageantry

18、The Piston Cup

19、The Big Race