本次推文分享一下snoRNAs方向基礎科研發文思路，文章發表在ONCOGENE上，該期刊屬於Nature子刊，屬於業內老牌期刊，影響因子：7.971，中科院最新分割槽：1區，屬於非OA期刊。

參考範文題目：

NOP10 predicts lung cancer prognosis and its associated small nucleolar RNAs drive proliferation and migration

研究背景：

Non-small cell lung cancer (NSCLC) is the leading cause of cancer death worldwide underlining the urgent need for new biomarkers and therapeutic targets for this disease. Long noncoding RNAs are critical players in NSCLC but the role of small RNA species is not well understood.

研究方法及結果：

In the present study, we investigated the role of H/ACA box small nucleolar RNAs (snoRNAs) and snoRNA-bound ribonucleoproteins (snoRNPs) in the tumorigenesis of NSCLC. H/ACA box snoRNPs including the NOP10 core protein were highly expressed in NSCLC. High levels of either NOP10 mRNA or protein were associated with poor prognosis in NSCLC patients. Loss of NOP10 and subsequent reduction of H/ACA box snoRNAs and rRNA pseudouridylation inhibited lung cancer cell growth, colony formation, migration, and invasion. A focused CRISPR/Cas9 snoRNA knockout screen revealed that genomic deletion of SNORA65, SNORA7A, and SNORA7B reduced proliferation of lung cancer cells. In line, high levels of SNORA65, SNORA7A, and SNORA7B were observed in primary lung cancer specimens with associated changes in rRNA pseudouridylation. Knockdown of either SNORA65 or SNORA7A/B inhibited growth and colony formation of NSCLC cell lines.

研究結論：

Our data indicate that specific H/ACA box snoRNAs and snoRNA-associated proteins such as NOP10 have an oncogenic role in NSCLC providing new potential biomarkers and therapeutic targets for the disease.

分析思路：

1、確定snoRNP複合蛋白NOP10在NSCLC中高表達，並與不良預後相關

2、確定敲減NOP10會損害NSCLC細胞的增殖，集落形成，遷移和侵襲

3、確定H / ACA box snoRNA在NSCLC患者樣品中高表達，可被敲減NOP10消耗

4、確定H / ACA box snoRNA是NSCLC細胞生長所必需的

5、確定SNORA65、7A和7B是肺癌細胞生長和增殖所必需的

6、SNORA65、7A和7B的下調既不會影響細胞週期程序，也不會影響NSCLC細胞的凋亡